Our prototypes showed that the bioprinted dermal structure successfully served as a platform for FDPC aggregation. In vivo like expression profile was maintained during the experiment. (Lost in 2D culture of dermal papilla cells). The polarization between the microwell and surface environment is indicating a dynamic environment. That the FDPCs had a clear effect on the expression markers of EKs, which exerted a pattern of markers, was only noted during co-culture with dermal papilla cells. Hair follicle-like structures are indicating that the model has the potential to modulate into a functional artificial fur /hair tissue. Conclusively a cruelty free model was established to bio print a combined fabric of fur and inseparably attached collagen tissues.
FIG. 1: Day 33, bioprinted Follicle -staining of K75 (red) with nucleuses counter stained with DAPI (blue). Cluster rotated to illustrate the configuration in different angles. Scale bar 100µm.
FIG. 2-8: Brightlight microscopy and DAPI staining.