The bioprinted prototypes showed that the dermal structure successfully served as a platform for HFDPC aggregation. In vivo like expression profile maintained during the experiment. (Lost in 2D culture of dermal papilla cells). Polarization between the microwell and surface environment, indicating a dynamic environment. That the HFDPCs had a clear effect on the expression markers of HEKs, which exerted a pattern of markers only noted during co-culture with dermal papilla cells. Hair follicle-like structures, indicating that the model has the potential to modulate into a functional artificial fur /hair model. Furthermore a cruelty free model is established to bio print a combined fabric of fur and inseparably attached collagen.
FIG. 1: Day 33, bioprinted Follicle -staining of K75 (red) with nucleuses counter stained with DAPI (blue). Cluster rotated to illustrate the configuration in different angles. Scale bar 100µm.
FIG. 2-8: Brightlight microscopy and DAPI staining.